Results
| PMID | 16311290 |
| Gene Name | HLA-G |
| Condition | Peritoneal endometriosis |
| Association |
Associated |
| Population size | 68 |
| Population details | 68 (archived tissue blocks from peritoneal endometriotic lesions (n = 15) and eutopic endometrium (n = 12) were evaluated for extent of protein immunostaining, and (ii) eutopic endometrial biopsies from women without (n = 17) and with (n = 24) endometrios |
| Sex | Female |
| Associated genes | HLA-G |
| Other associated phenotypes |
Peritoneal endometriosis |
|
Hum Reprod. 2006 Apr;21(4):864-9. Epub 2005 Nov 25. Barrier, B F| Kendall, B S| Ryan, C E| Sharpe-Timms, K L Department of Obstetrics, Gynecology and Women's Health, Division of Reproductive and Perinatal Research, University of Missouri-Columbia, Missouri 65212, USA. barrierb@health.missouri.edu BACKGROUND: HLA-G is a major histocompatability antigen with documented immune-regulatory function. Various epithelial cancers and tissue allografts have been noted to express HLA-G, which is postulated to aid in their escape from immunosurveillance. We evaluated peritoneal endometriosis and eutopic endometrium for the expression of HLA-G protein and gene transcript. METHODS: Two experiments were performed: (i) archived tissue blocks from peritoneal endometriotic lesions (n = 15) and eutopic endometrium (n = 12) were evaluated for extent of protein immunostaining, and (ii) eutopic endometrial biopsies from women without (n = 17) and with (n = 24) endometriosis, and peritoneal endometriotic lesions (n = 14) were evaluated for presence of RNA transcript by in situ hybridization. RESULTS: HLA-G protein localized in the glandular epithelium of 14 of 15 (93.3%) peritoneal endometriotic lesions, but not in stromal cells. HLA-G protein staining was absent in endometrial biopsies (n = 12). HLA-G gene transcript localized to the glandular epithelium in 13 of 14 (92.8%) peritoneal endometriotic lesions. HLA-G transcript was never observed in eutopic endometrium, regardless of cycle stage or whether from women with (n = 24) or without (n = 18) endometriosis. CONCLUSIONS: HLA-G is expressed by endometriotic glandular epithelium but not by eutopic endometrium under normal conditions. Differential expression of HLA-G suggests that peritoneal inflammation or cellular stress may up-regulate mechanisms to promote ectopic endometrial survival. Mesh Terms: Biopsy| Endometriosis/*immunology/pathology| Endometrium/*immunology/pathology| Epithelium/immunology/pathology| Female| HLA Antigens/genetics/*metabolism| HLA-G Antigens| Histocompatibility Antigens Class I/genetics/*metabolism| Humans| Immunohi |
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